Firefighting spokesperson Edwin Villagran said a multi-vehicle crash sent the bus off the road and into the steep gorge below the bridge before dawn. Others were seriously injured.
The bus fell 115 feet (35 meters) into a sewage-polluted stream. It landed upside down and half-submerged.
The bus had come from Progreso, northeast of the capital. Volunteer firefighting spokesman Óscar Sánchez said children were among the victims.
President Bernardo Arévalo offered his condolences and declared a day of national mourning.
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